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fetal liver kinase 1 flk1 protein (R&D Systems)

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R&D Systems fetal liver kinase 1 flk1 protein

Shear force induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. ( A - G ) Real-time PCR analysis of ANG1 , CD31 , EPCR , e-selectin , <t>FLK1</t> , TIE2 , and VEGF mRNA expression after 24-hour application of shear force at the magnitudes of 1.5 dyn/cm 2 (0.15 Pa) and 5 dyn/cm 2 (0.5 Pa). ( H - I ) The expression of CD31 and FLK1 proteins was detected by ELISA analysis. Data were statistically analyzed by Mann-Whitney U test. (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Fetal Liver Kinase 1 Flk1 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fetal+liver+kinase+1+flk1+protein/Recombinant+Human+VEGFR2%2FKDR%2FFlk-1+Fc+Avi-tag+Protein%2C+CF/pmc12284249-198-0-14
Average 93 stars, based on 1 article reviews

fetal liver kinase 1 flk1 protein - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells"

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

Journal: Scientific Reports

doi: 10.1038/s41598-025-12323-w

Figure Legend Snippet: Shear force induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. ( A - G ) Real-time PCR analysis of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF mRNA expression after 24-hour application of shear force at the magnitudes of 1.5 dyn/cm 2 (0.15 Pa) and 5 dyn/cm 2 (0.5 Pa). ( H - I ) The expression of CD31 and FLK1 proteins was detected by ELISA analysis. Data were statistically analyzed by Mann-Whitney U test. (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Techniques Used: Shear, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Extracellular ATP (eATP) induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. HPDLSCs were treated with various concentrations of eATP for 24 h. Expression of endothelial differentiation and angiogenic markers by HPDLCs were examined after eATP treatment. ( A - G ) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF were analyzed by real-time PCR. (H-I) Protein expression of CD31 and FLK1 were measured by ELISA. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Figure Legend Snippet: Extracellular ATP (eATP) induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. HPDLSCs were treated with various concentrations of eATP for 24 h. Expression of endothelial differentiation and angiogenic markers by HPDLCs were examined after eATP treatment. ( A - G ) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF were analyzed by real-time PCR. (H-I) Protein expression of CD31 and FLK1 were measured by ELISA. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

P 2 Y 1 receptor activation is associated with eATP-induced endothelial differentiation and angiogenesis of HPDLSCs. HPDLSCs were pre-treated with MRS 2179 30 min before the 24-hour eATP application. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Figure Legend Snippet: P 2 Y 1 receptor activation is associated with eATP-induced endothelial differentiation and angiogenesis of HPDLSCs. HPDLSCs were pre-treated with MRS 2179 30 min before the 24-hour eATP application. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Techniques Used: Activation Assay, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

Inositol 1-4-5 triphosphate (IP3) regulates eATP-induced endothelial differentiation and angiogenic marker expression by HPDLSCs. Pre-treatment with IP3 inhibitor (100 µg/ml Heparan sulfate) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD. *,**, and ***represent P <0.05, P <0.001 and P <0.0001, respectively.

Figure Legend Snippet: Inositol 1-4-5 triphosphate (IP3) regulates eATP-induced endothelial differentiation and angiogenic marker expression by HPDLSCs. Pre-treatment with IP3 inhibitor (100 µg/ml Heparan sulfate) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD. *,**, and ***represent P <0.05, P <0.001 and P <0.0001, respectively.

Techniques Used: Marker, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

The involvement of intracellular calcium signaling in eATP-induced endothelial differentiation and angiogenic markers expression by HPDLSCs. Pre-treatment with intracellular calcium mobilization inhibitor (50 µM TMB8) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD.*,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Figure Legend Snippet: The involvement of intracellular calcium signaling in eATP-induced endothelial differentiation and angiogenic markers expression by HPDLSCs. Pre-treatment with intracellular calcium mobilization inhibitor (50 µM TMB8) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD.*,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

Enzyme-linked Immunosorbent Assay:

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells.
Article Snippet: .. Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems. ..

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells
Article Snippet: .. Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems. ..

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Journal: Scientific Reports

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

doi: 10.1038/s41598-025-12323-w

Figure Lengend Snippet: Shear force induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. ( A - G ) Real-time PCR analysis of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF mRNA expression after 24-hour application of shear force at the magnitudes of 1.5 dyn/cm 2 (0.15 Pa) and 5 dyn/cm 2 (0.5 Pa). ( H - I ) The expression of CD31 and FLK1 proteins was detected by ELISA analysis. Data were statistically analyzed by Mann-Whitney U test. (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Article Snippet: Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems.

Techniques: Shear, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

doi: 10.1038/s41598-025-12323-w

Figure Lengend Snippet: Extracellular ATP (eATP) induced the expression of endothelial differentiation and angiogenic markers by HPDLSCs. HPDLSCs were treated with various concentrations of eATP for 24 h. Expression of endothelial differentiation and angiogenic markers by HPDLCs were examined after eATP treatment. ( A - G ) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF were analyzed by real-time PCR. (H-I) Protein expression of CD31 and FLK1 were measured by ELISA. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Article Snippet: Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Scientific Reports

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

doi: 10.1038/s41598-025-12323-w

Figure Lengend Snippet: P 2 Y 1 receptor activation is associated with eATP-induced endothelial differentiation and angiogenesis of HPDLSCs. HPDLSCs were pre-treated with MRS 2179 30 min before the 24-hour eATP application. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test followed by Dunn’s multiple comparison tests (n=6) *,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Article Snippet: Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems.

Techniques: Activation Assay, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Scientific Reports

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

doi: 10.1038/s41598-025-12323-w

Figure Lengend Snippet: Inositol 1-4-5 triphosphate (IP3) regulates eATP-induced endothelial differentiation and angiogenic marker expression by HPDLSCs. Pre-treatment with IP3 inhibitor (100 µg/ml Heparan sulfate) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD. *,**, and ***represent P <0.05, P <0.001 and P <0.0001, respectively.

Article Snippet: Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems.

Techniques: Marker, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Scientific Reports

Article Title: Shear stress-triggered adenosine triphosphate regulates angiogenic properties of human periodontal ligament stem cells

doi: 10.1038/s41598-025-12323-w

Figure Lengend Snippet: The involvement of intracellular calcium signaling in eATP-induced endothelial differentiation and angiogenic markers expression by HPDLSCs. Pre-treatment with intracellular calcium mobilization inhibitor (50 µM TMB8) was applied to human periodontal ligament cells 30 min before 200 µM eATP 24 h-treatment. (A-G) mRNA expression of ANG1 , CD31 , EPCR , e-selectin , FLK1 , TIE2 , and VEGF was analyzed using real-time PCR. (H-I) the protein expression of CD31 and FLK1 were evaluated using ELISA analysis. Data were statistically analyzed using Kruskal Wallis test by Dunn’s comparison test (n=6). The values are presented as the mean values±SD.*,**, and *** represent P <0.05, P <0.001 and P <0.0001, respectively.

Article Snippet: Fetal liver kinase-1 (FLK1) protein was measured by human VEGFR2/KDR (FLK1) Quantikine ELISA Kit (R&D systems.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Comparison

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fetal liver kinase 1 flk1 protein (R&D Systems)

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